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Regulation of apoptosis by ubiquitination
Nevertheless, to ascertain the success of this experimental design, the diluted cell lysates were also monitored for the yield of pure protein post-affinity purification (Fig
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The reaction was followed by measuring the formation of 1 mol NADH per consumed mol of TMABA photometrically at 340 nm ( NADH = 6,220 M 1 cm 1 , d = 1 cm, 30C)
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