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After centrifugation, 300 L PBS was added to resuspend cells, followed by the addition of 700 L pre-cooled anhydrous ethanol drop by drop, and incubated at 4 for 1824 h

To verify this, KGN cells overexpressing YTHDF1 were treated with the protein translation inhibitor CHX

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DAPI at 300 nM concentration in PBS was added for 5 min incubation