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Mechanistic studies to better understand the molecular targets and pathways involved in peptide action could help identify potential adverse effects and inform safer clinical use

AOD-9604 (Anti-Obesity Drug 9604) is a modified peptide fragment consisting of the terminal 15 amino acids of human growth hormone (positions 176-191) with a tyrosine residue added at the N-terminus for stability

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The Orbitrap full-scan MS spectra from m/z 3501800 were acquired at a resolution of 60,000 after accumulation to a target value of 3e 6

The rate of TNB formation is measured spectrophotometrically at 412 nm