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Immunofluorescence assay To detect the subcellular localization of SIRT5 and BCAT1, cells were fixed with 4% paraformaldehyde for at room temperature 20 min and permeabilized with phosphate-buffered saline (PBS) containing 0.5% Triton-X

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The absorption gap: Oral glutathione must survive stomach acid, intestinal enzymes, and first-pass liver metabolism before entering systemic circulation